ucircfull¶
About¶
The ucircfull package is a UMI-guided tool for identifying and quantifying circRNAs from UMI-tagged full-length circRNA sequencing (ucircFL-seq) data. It is designed to handle the challenges of low read depth and high sequencing error rates in ucircFL-seq data, and to provide accurate quantification of circRNA abundance.
Pipeline¶
Usage overview¶
Usage: ucircfull [--help] [--version] {circ_call,clust_umi,extract_umi}
Optional arguments:
-h, --help shows help message and exits
-v, --version prints version information and exits
Subcommands:
circ_call circRNA identification.
clust_umi UMI clusting guided consensus generation.
extract_umi Extract UMI sequence and identify strand from ucircFL-seq raw fastq